Mycelial inhibition of Trichoderma spp. isolated from the cultivation of Pleurotus ostreatus with an extract of Pycnoporus sp.
DOI:
https://doi.org/10.21829/abm127.2020.1537Keywords:
Disease control, edible mushrooms, lignocellulosic substrateAbstract
Background and Aims: The production of edible fungi is affected by bacterial, fungal and viral diseases, which very often cause large losses. In the production of mushrooms of the genus Pleurotus, the fungi of Trichoderma spp. represent a serious problem of contamination and although there are some chemical compounds that control the infection, they are not entirely safe for human consumption. As a consequence, alternatives are being searched for through biotechnology, such as the one presented in this paper.
Methods: Strains of fungi of the genus Trichoderma were isolated from the substrate where Pleurotus ostreatus was being cultivated. These were identified
morphologically and molecularly, followed by tests to inhibit the growth of Trichoderma strains in both agar and wheat straw, using a cetonic extract of the
dehydrated fruiting body of Pycnoporus sp.
Key results: Two strains of Trichoderma (T. pleuroti and T. atrobrunneum, belonging to the clade of T. harzianum) were isolated from infected substrate obtained in production modules of Pleurotus ostreatus located in Tlaquitenango and Cuernavaca, in the state of Morelos, Mexico. The effect of a cetonic extract of the fruiting body of Pycnoporus sp. on the mycelial growth of the isolated strains of Trichoderma was also evaluated, observing decrease in mycelial growth rate in Petri dish up to 72% and on lignocellulosic substrate both mycelial growth and sporulation were delayed up to 10 days.
Conclusions: The extract of Pycnoporus sp. could be an alternative to control the infection by Trichoderma spp. in mushroom cultures of the genus Pleurotus.
Downloads
References
Al-Fatimi, M., G. Schröder, H. Kreisel and U. Lindequist. 2013. Biological activities of selected basidiomycetes from Yemen. Die Pharmazie-An International Journal of Pharmaceutical Scien¬ces 68(3): 221-226.
Beyer, D. M., P. J. Wuest and J. J. Kremser. 2000. Evaluation of epidemiological factors and mushroom substrate characteristics influencing the occurrence and development of Trichoderma green mold. In: Van Griensven, L. J. L. D. (ed.). Science and Cul¬tivation of Edible Fungi. CRC Press. Maastricht, Netherlands. Pp. 633-640.
Calonge, F. D. 2011. Hongos medicinales. Ed. Mundi-Prensa. Soria, España. 130 pp.
Chaverri, P., F. Branco-Rocha, W. Jaklitsch, R. Gazis, T. Degenkolb and G. J. Samuels. 2015. Systematics of the Trichoderma harzianum species complex and the re-identification of commercial biocontrol strains. Mycologia 107(3): 558-590. DOI: https://doi.org/10.3852/14-147
Chen, X., C. P. Romaine, M. D. Ospina-Giraldo and D. J. Royse. 1999. A polymerase chain reaction-based test for the identification of Trichoderma harzianum biotypes 2 and 4, responsible for the worldwide green mold epidemic in cultivated Agaricus bisporus. Applied Microbiology and Biotechnology 52(2): 246-250. DOI: https://doi.org/10.1007/s002530051516
Choi, I. Y., S. B. Hong and M. C. Yadav. 2003. Molecular and morphological characterization of green mold, Trichoderma spp. isolated from oyster mushrooms. Mycobiology 31(2): 74-80. DOI: https://doi.org/10.4489/MYCO.2003.31.2.074
Danesh, Y. R., E. M. Goltapeh and H. Rohani. 2000. Identification of Trichoderma species causing green mold in button mushroom farms, distribution and their relative abundance. In: Van Griensven L. J. L. D. (ed.). Science and Cultivation of Edible Fungi. CRC Press. Maastricht, Netherland. Pp. 653-659.
Díaz-Godínez, G., M. Téllez-Téllez, A. Rodríguez, V. Obregón-Barbosa, M. L. Acosta-Urdapilleta and E. Villegas. 2016. Enzymatic, an¬tioxidant, antimicrobial, and insecticidal activities of Pleurotus pulmonarius and Pycnoporus cinnabarinus grown separately in an airlift reactor. BioResources 11(2): 4186-4200. DOI: https://doi.org/10.15376/biores.11.2.4186-4200
Gea, F. J. 2009. First report of Trichoderma pleurotum on oyster mushroom crops in Spain. Journal of Plant Pathology 91: 504.
Goltapeh, E. M. and Y. R. Danesh. 2000. Studies on interaction between Trichoderma species and Agaricus bisporus mycelium. In: Van Griensven, L. J. L. D. (ed.). Science and Cultivation of Edible Fungi. CRC Press. Maastricht, Netherlands. Pp. 661-666.
Guzmán, G. 1994. Los hongos en la medicina tradicional de Mesoamérica y de México. Revista Iberoamericana de Micología 11(3): 81-85.
Guzmán, G. 2003. Fungi in the Maya Culture: Past, Present and Future. In: Gómez-Pompa, A., M. F. Allen, S. L. Fedick and J. J. Jiménez-Osorio (eds.). The Lowland Maya Area. Food Products Press. Nueva York, USA. Pp. 315-325.
Guzmán, G. 2008. Diversity and use of traditional Mexican medicinal fungi. A review. International Journal of Medicinal Mushrooms 10(3): 209-217. DOI: https://doi.org/10.1615/IntJMedMushr.v10.i3.20
Hatvani, L., P. Sabolić, S. Kocsubé, L. Kredics, D. Czifra, C. Vágvölgyl, J. Kaliterna, D. Ivić and I. Kosalec. 2012. The first report on mushroom green mould disease in Croatia. Arhiv za Higijenu Rada i Toksikologiju 63(4): 481-486. DOI: https://doi.org/10.2478/10004-1254-63-2012-2220
Hatvani, L., Z. Antal, L. Manczinger, A. Szekeres, I. S. Druzhinina, C. P. Kubicek, A. Nagy, E. Nagy, C. Vágvölgyi and L. Kredics. 2007. Green mould diseases of Agaricus and Pleurotus spp. are caused by related but phylogenetically different Trichoderma species. Phytopathology 97(4): 532-537. DOI: https://doi.org/10.1094/PHYTO-97-4-0532
Index Fungorum. 2019. Index Fungorum database. http://www.indexfungorum.org/Names/Names.asp (consulted March, 2019).
Kim, J. W., S. Kwon and H. J. Kang. 1995. Studies on the pathogenic Pseudomonas causing bacterial disease of cultivated mushroom in Korea. Korean Journal Plant Pathology 11: 353-360.
Kim, K. Y., G. J. Lee, M. G. Ha, T. H. Lee and J. D. Lee. 2000. Intrageneric relationships of Trichoderma based on internal transcribed spacers and 5.8S rDNA nucleotide sequences. Mycobiology 28(1): 11-16. DOI: https://doi.org/10.1080/12298093.2000.1 2015716
Komoń-Zelazowska, M., J. Bissett, D. Zafari, L. Hatvani, L. Manczinger, S. Woo, M. Lorito, L. Kredics, C. P. Kubicek and S. I. Druzhini¬na. 2007. Genetically closely related but phenotypically divergent Trichoderma species cause green mold disease in oyster mushroom farms worldwide. Applied and Environmental Microbiology 73(22): 7415-7426. DOI: https://doi.org/10.1128/AEM.01059-07
Kredics, L., L. Hatvani, L. Antal, L. Manczinger, S. I. Druzhinina, P. C. Kubicek, A. Szekeres, A. Nagy, C. Vágvölgyi and E. Nagy. 2006. Green mold disease of oyster mushroom in Hungary and Transylvania. Acta Microbiologica et Immunologica Hungarica 53: 306-307.
Kredics, L., S. Kocsubé, L. Nagy, M. Komoń-Zelazowska, L. Manczinger, E. Sajben, A. Nagy, C. Vágvölgyi, C. P. Kubicek, I. S. Druzhinina and L. Hatvani. 2009. Molecular identification of Trichoderma species associated with Pleurotus ostreatus and natural substrates of the oyster mushroom. Microbiology Letters 300(1): 58-67. DOI: https://doi.org/10.1111/j.1574- 6968.2009.01765.x
Marik, T., P. Urbán, C. Tyagi, A. Szekeres, B. Leitgeb, M. Vágvölgyi, L. Manczinger, I. S. Druzhinina, C. Vágvölgyi and L. Kredics. 2017. Diversity profile and dynamics of peptaibols produced by green mould Trichoderma species in interactions with their hosts Agaricus bisporus and Pleurotus ostreatus. Chemistry and Biodiversity 14(6): e1700033. DOI: https://doi.org/10.1002/cbdv.201700033
Martínez-Alfaro, M. A., E. Pérez-Silva and E. Aguirre-Acosta. 1983. Etnomicología y exploraciones micológicas en la Sierra Norte de Puebla. Scientia Fungorum 2(18): 51-63.
NCBI. 2019. National Center for Biotechnology Information. https://blast.ncbi.nlm.nih.gov (consulted March, 2019).
Oei, P. 1991. Manual on mushroom cultivation: techniques, species and opportunities for commercial application in developing countries. TOOL Publications. Wageningen, Netherlands. 249 pp.
Park, M. S., K. S. Bae and S. H. Yu. 2004. Molecular and morphological analysis of Trichoderma isolates associated with green mold epidemic of oyster mushroom in Korea. Journal of Huazhong Agricultural University 23(1): 157-164.
Park, M. S., K. S. Bae and S. H. Yu. 2006. Two new species of Trichoderma associated with green mold of oyster mushroom cultivation in Korea. Mycobiology 34(3): 111-113. DOI: https://doi.org/10.4489/MYCO.2006.34.3.111
Qiu, Z., X. Wu, J. Zhang and C. Huang. 2017. High temperature enhances the ability of Trichoderma asperellum to infect Pleurotus ostreatus mycelia. PloS ONE 12(10): e0187055. DOI: https://doi.org/10.1371/journal.pone.0187055
Reyes, Q. C. K. 2013. Propiedades antifúngicas diferenciadas de extractos vegetales como estrategia potencial para la prevención, manejo y control del moho verde (Trichoderma spp.): En el cultivo de los hongos comestibles (Lentinula, Pleurotus). Tesis de doctorado. Colegio de Posgraduados (COLPOS). Texcoco, México.
Royse, D. J. 2014. A global perspective on the high five: Agaricus, Pleurotus, Lentinula, Auricularia & Flammulina. In: Singh, M. (ed.). Proceedings of the 8th International Conference on Mushroom Biology and Mushroom Products (ICMBMP8). ICAR-Directorate of Mushroom Research. New Delhi, India. 639 pp.
Samuels, G. L. and H. Prakash. 2015. Trichoderma: identification and agricultural applications (No. LC-0862). The American Phytopathological Society. Minnesota USA. 196 pp.
Sharma, S. R. and B. Vijay. 1996. Yield loss in Pleurotus ostreatus spp. caused by Trichoderma viride. Mushroom Research 5: 19-22.
Sobal, C. M., A. P. Morales, Q. M. Bonilla, S. W. Martínez and D. Martínez-Carrera. 2016. Biotecnología, innovación y desarrollo en la cadena de valor con base en los recursos genéticos de los hongos comestibles, medicinales y funcionales. In: Martínez-Carrera, D. and J. J. Ramírez (eds.). Ciencia, Tecnología e Innovación en el Sistema Agroalimentario de México. Colegio de Postgraduados-Academia Mexicana de Ciencias-Consejo Nacional de Ciencia y Tecnología-Universidad Popular Autónoma del Estado de Puebla-Instituto de Micología Neotropical Aplicada Press. Texcoco, México. Pp. 761-779.
Sobieralski, K., M. Siwulski, M. Kommon-Żelazowska, L. Błaszczyk, I. Sas-Golak and D. Frużyńska-Jóźwiak. 2012. Impact of Trichoderma pleurotum and T. pleuroticola isolates on yielding of Pleurotus ostreatus (FR.) Kumm. Journal of Plant Protection Research 52(1): 165-168. DOI: https://doi.org/10.2478/ v10045-012-0025-4
Soković, M. and L. J. van Griensven. 2006. Antimicrobial activity of essential oils and their components against the three major pathogens of the cultivated button mushroom, Agaricus bisporus. European Journal of Plant Pathology 116(3): 211-224. DOI: https://doi.org/10.1007/s10658-006-9053-0
StatSoft Inc. 2004. STATISTICA (data analysis software system) version 7.0. Tulsa, USA. https://statistica.software.informer.com/7.0/ (consulted March, 2019).
Téllez-Téllez, M., G. Díaz-Godínez and C. Sánchez. 2003. Physiology of colony Pleurotus pulmonarius grown on media overlaid with a cellophane membrane. Applied Microbiology and Biotechnology 63(2): 212-216. DOI: https://doi.org/10.1007/s00253-003-1387-3
Teoh, Y. P., M. M. Don and S. Ujang. 2011. Media selection for mycelia growth, antifungal activity against wood-degrading fungi, and GC-MS study by Pycnoporus sanguineus. BioResources 6(3): 2719-2731. DOI: https://doi.org/10.15376/biores.6.3.2719-2731
Wang, G., X. Cao, X. Ma, M. Guo, C. Liu, L. Yan and Y. Bian. 2016. Diversity and effect of Trichoderma spp. associated with green mold disease on Lentinula edodes in China. Microbiology Open 5(4): 709-718. DOI: https://doi.org/10.1002/mbo3.364
Woo, S. L., P. Di Benedetto, M. Senatore, K. Abadi, S. Gigante, I. Soriente, S. Ferraioli, F. Scala and M. Lorito. 2004. Identification and characterization of Trichoderma species aggressive to Pleurotus in Italy. Journal of Zhejiang University Agriculture and Life Sciences 30: 469-470.
Zehr, E. I. 1978. Methods for evaluating plant fungicides, nematicides, and bactericides. Prepared jointly by the American Phytopathological Society and the Society of Nematologists. American Phytopathological Society. St. Paul, Minnesota, USA. 141 pp.
Downloads
Published
How to Cite
-
Abstract7512
-
EPUB151
-
PDF554
-
XML44
Issue
Section
License
Copyright (c) 2019 Acta Botanica Mexicana
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish in this journal accept the following conditions:
In accordance with copyright legislation, Acta Botanica Mexicana recognizes and respects the moral rights of authors, as well as the ownership of the patrimonial right, which will be assigned to the journal for its dissemination in open access. Acta Botanica Mexicana does not charge authors for sending and processing articles for publication.
All the texts published by Acta Botanica Mexicana - without exception- are distributed under the Creative Commons 4.0 Attribution-Non Commercial License (CC BY-NC 4.0 International), which allows third parties to use what is published whenever they mention the authorship of the work and the first publication in this journal.
The authors can make other independent and additional contractual agreements for the non-exclusive distribution of the version of the article published in Acta Botanica Mexicana (for example, include it in an institutional repository or publish it in a book) as long as they clearly indicate that the work was published for the first time in Acta Botanica Mexicana
As a consequence, the copyeditors will request you, together with the galley review, to issue your Letter-Transfer of Ownership of Copyright form of the first publication duly completed and signed by the author(s). This letter should be sent by email in pdf file to the email: acta.botanica@inecol.mx (Letter-Transfer of Ownership of Copyright).
