Germination of cloud forest native shrubs with potential for restoration in central Veracruz, Mexico
DOI:
https://doi.org/10.21829/abm132.2025.2365Keywords:
Germination chamber, imbibition, propagation, seedsAbstract
Background and Aims: Seed germination studies are of great importance in conservation biology, restoration ecology, and the development of efficient propagation techniques. The use of native shrub species in restoration activities is scarce owing to lack of information on propagation, establishment, and general management. For this study, we assess the germination of eight shrub species native to the cloud forest by comparing seeds subjected to a pre-germinative treatment (imbibition) and seeds without treatment, under two germination conditions: greenhouse and germination chamber. We expect that under constant temperature conditions and exposure to a pre-germinative treatment, seeds will germinate more quickly and in higher proportions than seeds, exposed to temperature variations and without any pre-germinative treatment.
Methods: We setup a randomized experimental design consisting of 10 petri dishes containing 30 seeds per treatment (i.e., imbibition or control) in each germination condition (i.e., greenhouse or germination chamber). We recorded germination percentage, the number of days for initiation of germination, and t50.
Key results: Our results showed an important difference in the germination response between both germination conditions of the eight species evaluated. The optimal conditions for germination for most species were constant light and temperature of 25°C provided by the germination chamber, as this was associated with high rates of germination.
Conclusions: Understanding the seed germination process can provide valuable insights into the specific environmental conditions required for the successful propagation of the studied species. Furthermore, a thorough understanding of germination requirements can guide the development of more effective restoration techniques, as well as to inform conservation efforts by allowing managers to create strategies for optimal seed storage and handling and improve germination rates.
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Acero, A. M. and F. Cortés. 2014. Propagation of native species from the basin of La Vega River, Tunja, Boyacá, with potential for ecological restoration. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales 38: 195-205. DOI: https://doi.org/10.18257/raccefyn.76
Aguilar-Morales, M., H. Ochoterena-Booth and T. Terrazas. 2022. Seed morphology of Hamelieae with emphasis on the Deppea complex (Cinchonoideae, Rubiaceae). Plant Ecology and Evolution 155(1): 51-69. DOI: https://doi.org/10.5091/plecevo.84486 DOI: https://doi.org/10.5091/plecevo.84486
Aravind, J., S. Vimala, J. Radhamani, S. R. Jacob and S. Kalyani. 2023. Germinationmetrics: Seed Germination Indices and Curve Fitting. R package version 0.1.8.9000
Atondo-Bueno, E. J., M. Bonilla-Moheno and F. López-Barrera. 2018. Cost-efficiency analysis of seedling introduction vs. direct seeding of Oromunea mexicana for secondary forest enrichment. Forest Ecology and Management 409: 399-406. DOI: https://doi.org/10.1016/j.foreco.2017.11.028 DOI: https://doi.org/10.1016/j.foreco.2017.11.028
Augspurguer, C. K. 1979. Irregular rain cues and the germination and seedling survival of a Panamanian shrub (Hybanthus prunifolius). Oecologia 44(1): 53-59. DOI: https://doi.org/10.1007/BF00346397
Badano, E. I., C. G. Jones, L. A. Cavieres and J. P. Wright. 2006. Assessing impacts of ecosystem engineers on community organization: a general approach illustrated by effects of a high-Andean cushion plant. Oikos 115(2): 369-385. DOI: https://doi.org/10.1111/j.2006.0030-1299.15132.x DOI: https://doi.org/10.1111/j.2006.0030-1299.15132.x
Bandara, R. G., J. Finch, J. L. Walck, S. N. Hidayati and K. Havens. 2019. Germination niche breadth and potential response to climate change differ among three North American perennials. Folia Geobotanica 54: 5-17. DOI: https://doi.org/10.1007/s12224-019-09347-2 DOI: https://doi.org/10.1007/s12224-019-09347-2
Baskin, C. C. and J. M. Baskin. 2014. Seeds, ecology, biogeography and evolution of dormancy and germination. Academic Press. Kentucky, USA. Pp. 1586.
Bonfil, C. and I. Trejo. 2010. Plant propagation and the ecological restoration of mexican tropical deciduous forests. Ecological Restoration 28(3): 369-376. DOI: https://doi.org/10.3368/er.28.3.369 DOI: https://doi.org/10.3368/er.28.3.369
Brechú-Franco, A., Y. Jurado-González, G. Laguna-Hernández, A. Gómez-Campos and R. Fonseca-Juárez. 2018. Germination capacity and seedling survival of Moussonia deppeana (Gesneriaceae) from the montane cloud forests. Research Journal of Botany 13(1): 11-18. DOI: https://doi.org/10.3923/rjb.2018.11.18 DOI: https://doi.org/10.3923/rjb.2018.11.18
Burgos-Hernández, M., G. Castillo-Campos, M. Mata-Rosas, D. González, A. P. Vovides and J. Murguía-González. 2014. Seed germination of the wild banana Musa ornata (Musaceae). Seed Science and Technology 42(1): 16-27. DOI: http://doi.org/10.15258/sst.2014.42.1.02 DOI: https://doi.org/10.15258/sst.2014.42.1.02
Camargo-Ricalde, S. L., S. S. Dhillion and R. Grether. 2002. Community structure of endemic Mimosa species and environmental heterogeneity in semi-arid Mexican valley. Journal of Vegetation Science 13(5): 697-704. DOI: https://doi.org/10.1111/j.1654-1103.2002.tb02097.x DOI: https://doi.org/10.1111/j.1654-1103.2002.tb02097.x
Chaneton, E. J., C. Mazía and T. Kitzberger. 2010. Facilitation vs. apparent competition: Insect herbivory alters tree seedling recruitment under nurse shrubs in a steppe–woodland ecotone. Journal of Ecology 98(2): 488-497. DOI: https://doi.org/10.1111/j.1365-2745.2009.01631.x DOI: https://doi.org/10.1111/j.1365-2745.2009.01631.x
Delgado-Paredes, G. E., M. J. Kato, N. Vásquez-Dueñas, J. Minchala-Patiño and C. Rojas-Idrogo. 2012. Cultivo de tejidos de Piper sp. (Piperaceae): Propagación, organogénesis y conservación de germoplasma in vitro. Revista Colombiana de Biotecnología 14(2): 49-60.
Elgimabi, M. E. 2009. Improvement of propagation by hardwood cuttings with or without using plastic tunnel in (Hamelia patens). World Journal of Agricultural Sciences 5(5): 522-524.
Ellum, D. S. 2009. Floristic diversity in managed forests: Demography and physiology of understory plants following disturbance in southern New England forests. Journal of Sustainable Forestry 28(1-2): 132-151. DOI: http://dx.doi.org/10.1080/10549810802626431 DOI: https://doi.org/10.1080/10549810802626431
Escobar, D. and V. Cardoso. 2015. Germinación y latencia de semillas de Miconia chartacea (Melastomataceae), en respuesta a luz, temperatura y hormonas vegetales. Revista de Biología Tropical 63(4): 1169-1184. DOI: https://doi.org/10.15517/rbt.v63i4.17955
Fernández-Sánchez, L., C. Mancipe-Murillo and M. Calderón-Hernández. 2020. Evaluación de dos métodos de propagación para la conservación ex situ de tres melastomatáceas altoandinas. Caldasia 42(1): 129-141. DOI: http://dx.doi.org/10.15446/caldasia.v42n1.75373 DOI: https://doi.org/10.15446/caldasia.v42n1.75373
Figueroa, J., J. Armesto and J. Hernández. 1996. Estrategias de germinación y latencia de semillas en especies del bosque templado de Chiloé, Chile. Revista Chilena de Historia Natural 69: 243-251.
Funes, G., S. Díaz and P. Venier. 2009. La temperatura como principal determinante de la germinación en especies del Chaco seco de Argentina. Ecología Austral 19: 129-138.
Garwood, N. C. 1983. Seed germination in a seasonal tropical forest in Panama: A community study. Ecological Monographs 53(2): 159-181. DOI: https://doi.org/10.2307/1942493 DOI: https://doi.org/10.2307/1942493
Gelviz-Gelvez, S. M., N. P. Pavón, P. Illoldi-Rangel and C. Ballesteros-Barrera. 2015. Ecological niche modeling under climate change to select shrubs for ecological restoration in Central Mexico. Ecological Engineering 74: 302-309. DOI: https://doi.org/10.1016/j.ecoleng.2014.09.082 DOI: https://doi.org/10.1016/j.ecoleng.2014.09.082
Gelviz-Gelvez, S. M., N. P. Pavon, J. Flores, F. Barragán and H. Paz. 2020. Germination of seven species of shrubs in semiarid central Mexico: Effect of drought and seed size. Botanical Sciences 98(3): 464-472. DOI: https://doi.org/10.17129/botsci.2537 DOI: https://doi.org/10.17129/botsci.2537
Godefroid, S. and A. Van de Vyver. 2020. Seed germination ecology of Viola calaminaria, an endangered metallophyte with a narrow distribution. Plant Species Biology 35: 89-96. DOI: https://doi.org/10.1111/1442-1984.12259 DOI: https://doi.org/10.1111/1442-1984.12259
Godoi, S. and M. Takaki. 2007. Seed germination in Miconia theaezans (Bonpl.) Cogniaux (Melastomataceae). Brazilian Archives of Biology Technology 50(4): 571-578. DOI: http://dx.doi.org/10.1590/S1516-89132007000400002 DOI: https://doi.org/10.1590/S1516-89132007000400002
Gutiérrez, J. R. and F. A. Squeo. 2004. Importancia de los arbustos leñosos en los ecosistemas semiáridos de Chile. Ecosistemas 13: 1-14.
Herrera, C., C. Carrillo, H. González, S. Carrillo, V. Peña and N. García. 2011. Tratamientos químicos para recuperar la germinación en semillas de cebolla. Revista Chapingo Serie Horticultura 17: 63-72.
Hillebrand, H., D. M. Bennett and M. W. Cadotte. 2008. Consequences of dominance: a review of evenness effects on local and regional ecosystem processes. Ecology 89(6): 1510-1520. DOI: https://doi.org/10.1890/07-1053.1 DOI: https://doi.org/10.1890/07-1053.1
Hills, P. N. and J. van Staden. 2003. Thermoinhibition of seed germination. South African Journal of Botany 69(4): 455-461. DOI: https://doi.org/10.1016/S0254-6299(15)30281-7. DOI: https://doi.org/10.1016/S0254-6299(15)30281-7
Jurado, Y. 2013. Propagación sexual y asexual de Moussonia deppeana (tlachichinole), especie medicinal. Tesis de licenciatura. Licenciatura en Biología, Universidad Nacional Autónoma de México. D.F., Mexico. 95 pp.
Martínez, N. and E. De la Barrera. 2020. Ecofisiología de la germinación de tres malezas efímeras periurbanas en Morelia, Michoacán, México. La Granja: Revista de Ciencias de la Vida 31(1): 47-55. DOI: http://dx.doi.org/10.17163/lgr.n31.2020.03 DOI: https://doi.org/10.17163/lgr.n31.2020.03
Martínez-Calderón, V. M., J. Sosa-Ramírez, J. J. Luna-Ruíz, D. R. Pérez-Salicrup and M. H. Sandoval-Ortega. 2023. Effect of storage and pre-germination treatments on seeds of Arbutus xalapensis from north-central Mexico. New Forests 54: 1119-1130. DOI: https://doi.org/10.1007/s11056-022-09957-3 DOI: https://doi.org/10.1007/s11056-022-09957-3
Montaño-Arias, N. M., S. L. Camargo-Ricalde, R. García-Sánchez and A. Monroy-Ata. 2008. Micorrizas Arbusculares en Ecosistemas áridos y Semiáridos. Mundi-Prensa S.A. de C.V. Instituto Nacional de Ecología-Secretaría de Medio Ambiente y Recursos Naturales. México, D.F., México. 460 pp.
Montejo, L. A., J. A. Sánchez and B. Muñoz. 2002. Efectos de la temperatura sobre la germinación de plantas aromáticas. Acta Botánica Cubana 190: 28-30.
Montes-Hernández, B. and F. López-Barrera. 2013. Seedling establishment of Quercus insignis: A critically endangered oak tree species in southern Mexico. Forest Ecology and Management 310: 927-934. DOI: http://dx.doi.org/10.1016/j.foreco.2013.09.044 DOI: https://doi.org/10.1016/j.foreco.2013.09.044
Muñoz, B., J. Sánchez and W. Almaguer. 2004. Germinación, dormancia y longevidad potencial de las semillas de Guazuma ulmifolia. Pastos y Forrajes 27: 25-32.
Núñez-Cruz, A., J. A. Meave and C. Bonfil. 2018. Reproductive phenology and seed germination in eight tree species from a seasonally dry tropical forest of Morelos, Mexico: implications for community-oriented restoration and conservation. Tropical Conservation Science 11: 1-14. DOI: https://doi.org/10.1177/1940082917749946 DOI: https://doi.org/10.1177/1940082917749946
Odusanya, T. I., C. O. Owolabi, O. M. Olosunde, G. J. Bodunde and J. N. Odedina. 2019. Propagation and seedling growth of some species used as ornamental hedges in landscape design. Ornamental Horticulture 25(4): 383-389. DOI: http://dx.doi.org/10.1590/2447-536x.v25i4.2053 DOI: https://doi.org/10.1590/2447-536x.v25i4.2053
Oliveira-Silveira, F. A., P. Oliveira Mafia, J. P. Lemos-Filho and G. Wilson Fernandes. 2012. Species-specific outcomes of avian gut passage on germination of Melastomataceae seeds. Plant Ecology and Evolution 145(3): 350-355. DOI: http://dx.doi.org/10.5091/plecevo.2012.706 DOI: https://doi.org/10.5091/plecevo.2012.706
Pérez-Cadavid, A., O. R. Rojas-Soto and M. Bonilla-Moheno. 2018. Effect of seed ingestion by birds on the germination of understorey species in cloud forest. Revista Mexicana de Biodiversidad 89(2018): 1167-1175. DOI: http://dx.doi.org/10.22201/ib.20078706e.2018.4.2612 DOI: https://doi.org/10.22201/ib.20078706e.2018.4.2612
Pérez-Hernández, I., S. Ochoa-Gaona, G. Vargas-Simón, M. Mendoza-Carranza and N. A. González-Valdivia. 2011. Germinación y supervivencia de seis especies nativas de un bosque tropical de Tabasco, México. Madera y Bosques 17(1): 71-91. DOI: https://doi.org/10.21829/myb.2011.1711155 DOI: https://doi.org/10.21829/myb.2011.1711155
Pérez Mendoza, C., G. Carrillo Castañeda, E. Vidal Lezama and E. Ortiz García. 2016. Efecto de la imbibición en la calidad fisiológica de semillas de jitomate. Revista Mexicana de Ciencias Agrícolas 7(7): 1765-1773. DOI: https://doi.org/10.29312/remexca.v7i7.169 DOI: https://doi.org/10.29312/remexca.v7i7.169
R Core Team. 2022. R: A language and environment for statistical computing, ver. 4.4.2. R Foundation for Statistical Computing. Vienna, Austria. https://www.R-project.org/ (consulted December, 2023)
Román, F., R. D. Liones, A. Sautu, J. Deago and J. S. Hall. 2012. Guía para la propagación de 120 especies de árboles nativos de Panamá y el Neotrópico. Environmental Leadership Training Initiative and PRORENA. Cali, Colombia. 162 pp.
Sánchez, J., A. J. A. Mejía, A. Hernández, A. Peña and C. Carballo. 2007. Acondicionamiento osmótico de semillas de tomate de cáscara. Agricultura Técnica en México 33: 115-123.
Sánchez, J. A., L. Montejo, A. Gamboa, D. Albert-Puentes and F. Hernández. 2015. Germinación y dormancia de arbustos y trepadoras del bosque siempreverde de la Sierra del Rosario, Cuba. Pastos y Forrajes 38: 11-28.
Sierra-Escobar, J. A., Ma. A. Quijano Marín, D. Marín-Henao, D. Salazar Suaza and M. V. García Garcés. 2020. Especies pioneras, persistentes y ensayos de germinación en bosques montanos de la cordillera central, Colombia. Ciencia en Desarrollo 11(2): 7-24. DOI: https://doi.org/10.19053/01217488.v11.n2.2020.10645 DOI: https://doi.org/10.19053/01217488.v11.n2.2020.10645
Silva, M. H. L., R. C. L. Costa, A. K. S. Lobato, C. F. Oliveira Neto and H. D. Laughinghouse IV. 2007. Effect of temperature and water restriction on Piper aduncum L. seed germination. Journal of Agronomy 6(3): 472-475. DOI: http://dx.doi.org/10.3923/ja.2007.472.475 DOI: https://doi.org/10.3923/ja.2007.472.475
Silveira, F. A. O., G. W. Fernandes and J. P. Lemos-Filho. 2013. Seed and Seedling Ecophysiology of Neotropical Melastomataceae: Implications for Conservation and Restoration of Savannas and Rainforests. Annals of the Missouri Botanical Garden 99(1): 82-99. DOI: http://dx.doi.org/10.3417/2011054 DOI: https://doi.org/10.3417/2011054
Suárez, D. and L. M. Melgarejo. 2010. Biología y germinación de semillas. In: Melgarejo, L. M. (ed.). Experiments in Plant Physiology. Universidad Nacional de Colombia. Bogotá, Colombia. Pp. 89-96.
Toledo-Aceves, T., A. L. Trujillo-Miranda and F. López-Barrera. 2021. Tree regeneration in active and passive cloud forest restoration: Functional groups and timber species. Forest Ecology and Management 489(1): 119050. DOI: http://dx.doi.org/10.1016/j.foreco.2021.119050 DOI: https://doi.org/10.1016/j.foreco.2021.119050
Toledo-Aceves, T., F. López-Barrera, V. Vázquez-Reyes and S. Günter. 2022. Restoration of tropical montane cloud forest in bracken dominated pastures: The role of nurse shrubs. Forest Ecology and Management 508: 120055. DOI: https://doi.org/10.1016/j.foreco.2022.120055 DOI: https://doi.org/10.1016/j.foreco.2022.120055
Varela, S. and V. Arana. 2010. Latencia y germinación de semillas. Tratamientos pregerminativos. Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria. Bariloche. Buenos Aires, Argentina. Pp. 10.
Vázquez-Yanes, C. and A. I. Batis. 1996. Adopción de árboles nativos valiosos para la restauración ecológica y la reforestación. Botanical Sciences 58: 75-84. DOI: https://doi.org/10.17129/botsci.1488 DOI: https://doi.org/10.17129/botsci.1488
Velázquez-Escamilla, T. L., C. Díaz-Castelazo, B. Ruiz-Guerra and N. Velázquez-Rosas. 2019. Síndromes de dispersión de semillas en comunidades de bosque mesófilo de montaña, en la región centro de Veracruz, México. Botanical Sciences 97(4): 568-578. DOI: https://doi.org/10.17129/botsci.2095 DOI: https://doi.org/10.17129/botsci.2095
Villaseñor, J. L. 2016. Checklist of the native vascular plants of Mexico. Revista Mexicana de Biodiversidad 87(2016): 559-902. DOI: https://doi.org/10.1016/j.rmb.2016.06.017 DOI: https://doi.org/10.1016/j.rmb.2016.06.017
Viveros, H., J. D. Hernández and M. V. Velasco. 2015. Análisis de semilla, tratamientos pregerminativos de Enterolobium cyclocarpum (Jacq.) Griseb. y su crecimiento inicial. Revista Mexicana de Ciencias Forestales 6(30): 52-65. DOI: https://doi.org/10.29298/rmcf.v6i30.207
Williams-Linera, G. 2012. El Bosque de niebla del centro de Veracruz: Ecología, historia y destino en tiempos de fragmentación y cambio climático. Xalapa, Veracruz, México: Instituto de Ecología, A.C. - Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO). Xalapa, México. 208 pp.
Williams-Linera, G., M. Toledo-Garibaldi and C. Gallardo Hernández. 2013. How heterogeneous are the cloud forest communities in the mountains of central Veracruz, Mexico? Plant Ecology 214: 685-701. DOI: https://doi.org/10.1007/s11258-013-0199-5 DOI: https://doi.org/10.1007/s11258-013-0199-5
Wittaker, R. H. 2012. Classification of Plant Communities. Springer Dordrecht. Ithaca, USA. 408 pp. DOI: https://10.1007/978-94-009-9183-5
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